Translational_Unit

Part:BBa_K118014:Design

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-08-19)


rbs+crtE (geranylgeranyl pyrophosphate synthase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 725
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The crtE BioBrick (BBa_I742151) had to be transferred from the non-compliant BABEL vector to a compliant pSB1A2 vector.


Source

The synthetic ribosome binding site was added by fusion PCR to the crtE gene, which was amplified from Pantoea ananatis DSMZ 30080 (ATCC 19321) genomic DNA.

References